PFCC Guidelines - Getting started in:

Flow Cytometry Analysis

(1) Read our Guidelines A to help prepare for your flow experiment.

(2) Getting Started with Full Spectrum Flow (coming soon)

Cell Sorting

(1) Read our Guidelines S to help prepare for your sorting experiment.

  • Basic FACS Buffer recipe (coming soon)
  • Basic recommendations for Collection Buffer recipes (coming soon)

(2) Use our helpful Cell Sorting Calculators to decide what is the appropriate time to book for your experiments.

(3)  Do your final check using the Cell Sorting Checklist

Mass Cytometry

[EXT] Mass Cytometry/CytOF (Helios) Resources by UChicago

Small Particle Analysis

PFCC Nanosight Guidelines (coming soon)

other coming: Recommended Sorting supplies, Guidelines for plate sorting. Do you have any requests? Submit your suggestion here: SUGGESTION BOX!

PFCC Instrument QuickGuides-Handouts

ANALYZERS

StartUp and Change over / Shutdown instructions

SORTERS

Change over / Shutdown instructions

Monitoring a sort on the:

 

OTHERS

  • Mass Cytometer "CyTOF / Helios" QuickGuide (coming soon)
  • "NanoSight NS300" QuickGuide (coming soon)
  • "Luminex 100" QuickGuide (coming soon)

Please note: due to the COVID-19 pandemic please review any additional safety and cleaning procedures on our COVID-19 support page.

Protocols and Recommendations

Cell Harvesting and Preparation

Worthington Tissue Dissociation Guide: Link to website

Cell Sorting Buffer Recipes (coming soon)

more coming soon

Flow Cytometry Staining

OMIPs: check this published Optimized Multicolor Immunofluorescence Panels for flow cytometry. These can provide you a starting point and alleviate the development time in need of the same or highly similar panels.

more coming soon

Common Applications of Flow Cytometry

RNA isolation from sorted cells - This paper has a detailed protocol for how to properly prepare a sorter for RNA isolation and sort cells with Trizol, RLT, and RNase inhibitor. It also includes an estimated yield of RNA from certain cell types.

Measurement and Characterization of Apoptosis by Flow Cytometry: [EXT] Reference [1] and [2]

PhosphoFlow

[EXT] 10x Genomics Guidelines for Sorting