New Users

All New users of PFCC are required to go through Onboarding. Additional training (if needed) is recommended before operating any of the self-use equipment in the Flow Core to make sure that every user is aware of the facility policies and instrument procedures. 

If you will use the instruments infrequently the Core offers Operator Assistance service.

If you are new to flow/mass cytometry or have general questions, we suggest you setup a consultation with the Core Staff first during Office Hours for help with experimental and panel design.

Please follow the steps below:

1. Set up your iLab Account

You must have an iLab account before signing up for Onboarding or utilizing services in the Parnassus Flow Cytometry Core. Click HERE and Sign Up for an account using your UCSF MyAccess credential; your PI/Lab Manager will receive a notification to approve your iLab account, and will need to grant access to the groups speedtypes as well. If you are experiencing any access issues please see our FAQ.

2. Schedule and attend Onboarding

Within iLab, start your PFCC onboarding process on Schedule Instrument tab and navigate to the Training category and register for the next available Onboarding Session. These are 1hr sessions held every other week, on Wednesdays 1-2pm, to introduce New Users to the PFCC Facility Policies.  If you are experiencing any scheduling issues on iLab please see our FAQ. [After completion, status: Onboarded]. After Onboarding, you will be able to book instruments (with assistance) and receive your lab's login credentials. Once you have your iLab and Lab Instrument Software Accounts, you may plan to schedule any services on iLab.

3. Additional Training

New users of PFCC who expect to operate any of the self-use equipment independently in the Flow Core are required to go through training, before scheduling any self-use sessions. Visit our website HERE for details on training options. 

4. Get Room Access

You will need to request access to the facilities where the instruments are located. Please click HERE to read the instructions on how to request access.

5. See and Review our Rules and Scheduling Policies

After you have been onboarded, and before your first time at PFCC, please read through and review the updated Facility Policies and Scheduling Rules.

Data Analysis

PFCC has three workstations accessible to all users for post-acquisition data analysis. Different software site licenses are also available for purchase through RRP. See here for options to analyze the data off the instruments.    

Acknowledgements

The Diabetes Research Center (DRC) subsidizes the cost for DRC members and financially supports project development for all users. Please inform us if you publish using data obtained from our facility. Your publications help support our center grant which assists in keeping this Flow Cytometry Facility running, more information here.

• Mention RRID:SCR_018206 and P30 DK063720 for all services
• 1S10OD018040-01 for using the CyTOF-Helios services
• 1S10OD021822-01 for using any of the FACSArias
• 1S10OD026940‐01 for using the Auroras
• 1S10OD025187-01 for using the MIBIscope