New Users

New users of PFCC are required to go through onboarding and additional training (if needed) before operating any of the self-use equipment in the Flow Core to make sure that every user is aware of the facility policies and instrument procedures. 

If you are new to flow/mass cytometry or have general questions, we highly suggest you setup a consultation with the Core Staff first during Office Hours to see what is possible.  We can help you with experimental and panel design as well.

*** Please be aware there are certain limitations and new guidelines due to COVID-19 pandemic. Please visit our for the latest information regarding the guidelines and support during the COVID-19 pandemic . **

Set up your iLab Account

You must have an iLab account before signing up for Onboarding or utilizing services in the Parnassus Flow Cytometry Core. Click HERE and Sign Up for an account using your UCSF MyAccess credential; your PI/Lab Manager will receive a notification to approve your iLab account, and will need to grant access to the groups speedtypes as well. If you are experiencing any access issues please see our FAQ.

Schedule and attend the Onboarding Session

Within iLab, start your PFCC onboarding process on Schedule Instrument tab and navigate to the Training category and register for the next available Onboarding Session. Seats are limited. These are 1hr sessions held weekly, every Monday 10-11AM, to introduce New Users to the PFCC Facility Policies.  If you are experiencing any scheduling issues on iLab please see our FAQ. [After completion, status: Onboarded]

 
Training is required for all Self-use instruments

New users of PFCC who expect to operate any of the self-use equipment in the Flow Core are required to go through training, or show their proficiency, before scheduling any self-use session. Visit our website HERE for details on each class. The Core also offers Assistance service if you are looking to run an experiment before completing your training. To save a seat for any of the training offered, Log In to iLab, on Schedule Instrument navigate to the Training category schedule the appropriate class for your training. Depending on your experience level you will sign up for some or all of the offered classes, it is highly recommended following the program in the order mentioned. [After completion status: Trained]

See and Review our Rules and Scheduling Policies

After you have been onboarded, read through and review the Facility Policies and Scheduling Rules.

Get your Lab Account

To access the aquisition software on any PFCC self-use equipment your lab must also have a Lab Instrument Account with us. The Flow Core will provide your lab login and password for the instruments after Onboarding. Once you have your iLab and Lab Instrument Accounts, you may schedule any hands-on training, service, and equipment utilization. 

Get Room Access

You will need to request access to the facilities where the instruments are located. Please click HERE to read the instructions on how to request access. If you are in need of the Operator Assisted Cell Sorting Service or Assistance to have Flow Core staff run your samples please submit the required Form and, contact us ahead if needed.

Data Analysis

PFCC has three workstations accessible to all users for post-acquistion data analysis. Different software site licenses are also available for purchase. See here for options to analyze the data off the instruments.    

Acknowledgements

The Diabetes Research Center (DRC) subsidizes the cost for DRC members and financially supports project development for all users. Please inform us if you publish using data obtained from our facility. Your publications help support our center grant which assists in keeping this Flow Cytometry Facility running. Please acknowledge DRC Center Grant NIH P30 DK063720.  If using the CyTOF2 "Charmander" please acknowledge Shared Instrument Grant 1S10OD018040-01.  If using any of the FACSArias please acknowledge Shared Instrument Grant 1S10OD021822-01. If using the Aurora please acknowledge Shared Instrument Grant 1S10OD026940‐01. If using the MIBIscope please acknowledge Shared Instrument Grant 1S10OD025187-01.

 

New Users Checklist

If unsure about the potential of PFCC technologies for your project, schedule a consultation with the Core staff.

1. Get your iLab account setup.

2. Schedule and attend the Onboarding Session, and any additional training if needed.

3. Confirm access to your Lab iLab Speedtypes, instrument privileges, and Lab Instrument Account

4. Get Room Keycard access

5. Schedule your Guidance during first self-use experiment, and Start Flowing.

6. Check your options for data analysis. If necessary, contact staff for data analysis interpretation.

7. If you publish with any of our instruments and services, please acknowledge the Core and inform us, see HERE for details.

 

External users

Please click here http://flow.ucsf.edu/new-users-externals