Flow Cytometry | Analyzers

In this section you will find information related with all the Flow Cytometry Analyzers and respective services currently available at the Parnassus Flow Cytometry Core, these are:

  1. Self analysis on the FACSCalibur
  2. Self analysis on the LSRII / Fortessa / X20
  3. Self analysis on the FACSymphony
  4. Self analysis on the Aurora
  5. Self analysis on the Luminex 200
  6. Self analysis on the Nanosight NS300

This LINK contains the configurations of all instruments in the Core. 

The Summary Table will provide a matrix of all the key features of each instrument. Click on the nickname link of a specific instrument to get a more detailed layout.  Tabs are also at the bottom listing the machine nicknames. It is essential for planning your experiments and seeing if an instrument can detect your specific markers. Please contact us if you have any questions or are in need of a specific filter set or setup for your experiments. 

Availability: 24 hours, 7 days a week.

Training:  All Self Analysis users need to fulfill Basic Training requirements. Click HERE.

Assistance:  If you run samples infrequently, it is recommended to request Assistance to have a Flow Core staff member run samples for you. Click HERE.

Rates:  Click HERE.

Scheduling:  Click HERE.

Data Analysis:  Raw data is in a FCS file format.  For analysis options, click HERE.

 

Self Analysis on the FACSCalibur

The BD FACSCalibur is a flow cytometer analyzer equipped with 2 lasers, generating data of up to 6 parameters for each event passing through the interrogation point.

Analysis occurs at the cuvette flow cell, increasing sensitivity and resolution, allowing detection of 2 relative physical properties as well as up to 4 different colors simultaneously. It can be run at 3 different sample flow rates and is capable of analysis rates of up to 20,000 events per second.  It is operated using CellQuest Pro Software and is available for all labs to use.

 

 

Self Analysis on the LSRII / Fortessa / X20

The BD LSRII, Fortessa, and X-20 class of instruments are flow cytometer analyzers equipped with 4 or more lasers, generating data of up to 20 parameters for each event passing through the interrogation point.

The LSRII, Fortessa, and X-20 appear different, but the operation and workflow are performed in the same manner on all instrument types.  Some are also equipped to take the High Throughput Sampler (HTS) for 96 and even 384 well type plates.  Analysis occurs at the cuvette flow cell, increasing sensitivity and resolution, allowing detection of 2 relative physical properties as well as up to 18 different colors simultaneously. It can be run at 3 different sample flow rates with a fine-tune adjustor and is capable of analysis rates of up to 40,000 events per second.  They are all operated using FACSDiva Software and are available for all labs to use.

 High-throughput sampler (HTS) is available in some of these instruments, and the Core provides a logistical training to help you run it.

 

 

Self Analysis on the FACSymphony

The BD LSRFortessa X-50 (also known as FACSymphony) is the flow cytometer analyzer equipped with 4, generating data of up to 30 parameters for each event passing through the interrogation point.

QC Instrument Settings

Flow Core / Trained Staff: Click here to update QC sheet

Self Analysis on the Aurora

Cytek's Spectral Flow Cytometer equipped with five excitation sources and 64 fluorescence emission detection channels, which provides measurement of the full visible spectrum for each fluorochrome. Automatic micro-sampling system (AMS) for sampling of standard 96-well format is available.

  • Laser Excitation sources: UV, 405, 488, 561, 640 nm
  • Detector modules: 16, 16, 14, 10, 8 channels respectively
  • AMS available to smaple cells from a U-bottom, V-bottom, or flat bottom standard-height 96-well plate
  • Direct volumetric measurement for cell counting
  • Microparticle detection (up 100nm) capabilities: Violet side scatter detector for thresholding, and several pulse processing properties

Self Analysis on the Luminex 100 - BioPlex 200

Available as walk-up platform, PFCC does not provide training for this instrument, please reachout to your kit vendor application scientist.

  • Multiplexing analyzer that can analyze up to 100 analytes per sample/well.
  • Automatic sampling from a 96-well plate.
  • Maintain samples at a constant temperature from 35°C to 55°C (95°F to 131°F)
  • Magnetic holder availabe for request

 

 

 

 

 

Self Analysis on the Nanosight NS300

nanosightMalvern's NanoSight NS300 detects particles from 10nm to 2000nm in size, counts and sizes small particles to define concentration in solution.

  • Detects particles from 10nm to 2000nm in size
  • Configurable with lasers: 488 nm or 532 nm, AND 500LP and 532LP

Common applications of the Nanosight include:

  • Exosomes, microvesicles and extracellular vesicles characterization.
  • Viral vaccine research and development.
  • Development of drug delivery systems.
  • Protein aggregation studies.
  • Nanotoxicology.
  • Be able to analyze samples based on size distribution and concentration of all types of Nano particles from 10nm to 2000nm in diameter in solution. It is operated using the Nanoparticle Tracking Analysis (NTA) software. Sample temperature is fully programmable by NTA.