PFCC Mass Cytometry Training

PFCC Mass Cytometry Training

Hands-on CyTOF Training

The type of training conducted is dependent on your needs.  If you plan to run during normal working hours (Monday-Friday from 8am-5pm), Basic Training will suffice.  Members of the Flow Core perform "CyTOF-Setup" during normal working hours based off the scheduled users. 

Resources:  Please view the manual before your scheduled training, as it will help you in understanding mass cytometry basics (Chapter 1, pages 4-21) and the software (Chapter 5, pages 94-101). 

1)  Recommended for new users of the CyTOF:  Helios User Guide V6.7  

2)  Get an account here as this will give you access to important resources and background info as well as the panel designer: http://www.dvssciences.com/login.php 

3)  See here for product support including the link for the Fluidigm Debarcoder software: https://www.fluidigm.com/singlearticles/how-barcoding-works

4)  See here for the link to the Nolan lab tools such as their debarcoder and normalization programs:  https://github.com/nolanlab

Basic Training Requirements:  We allow you to evaluate your skill level (Novice, Experienced), but if we observe you need additional help, you will be required to take supplementary training.  Choose from one of the 2 options:

  • Novice Training: is done on individual level and consists in performing a pilot experiment with your panel. You will learn how to stain (link to protocol), barcode and run your stained samples on the available Helios. Please email the staff at [email protected] and ask about training availability.
  • Experienced user: If you are a New User please sign up for the Onboarding Session at PFCC.