FEATURED WEBINAR:
An Optimized Method for Accurate Spread Measurement & its Use in Panel Design
Ask the Experts:
Mirko Corselli, PhD & Bob Balderas, MBA
Thurs, May 13, 2021 8:00 AM PST | 11:00 AM EST
When designing multicolor flow cytometry panels, assessment of fluorescence spillover is critical to prevent or minimize loss of resolution due to spreading error. The Spillover Spread Matrix (SSM) was developed as a powerful tool to monitor and compare instrument performance over time, especially in the setting of experiments standardized or calibrated across different instruments. SSM has also been widely adopted as a tool to guide panel design.
The intrinsic nature of SSM, i.e., independence from fluorochrome brightness, is a critical feature for instrument performance comparison but may lead to inaccurate spread prediction and consequent sub-optimal panel design. In this webinar, we will introduce a new, optimized method for a more precise spread quantification and specifically developed to aid panel design.
Key learnings:
- Benefits and limitation of SSM for multicolor flow cytometry panel design
- Impact of inaccurate spread prediction on data quality
- Generation of an optimized spread matrix for a more accurate spread assessment
MEET THE SPEAKERS
Bob Balderas, MBA
Distinguished Fellow, VP Biological Sciences,
VP Market Development
BD Biosciences
Mirko Corselli, PhD
Global Scientific Content Manager
BD Biosciences