Self Sorting on the SH800

SH800 pic1

Availability: 24 hours, 7 days a week. (Monday-Friday, 8am-7pm with "SH800 Setup.")

Overview:  The Sony SH800 is a walk-up flow cytometer cell sorter equipped with 4 lasers, generating data of up to 8 parameters for each event passing through the interrogation point, and allows the collection of up to 2 different populations. 

This sorter can detect 2 relative physical properties as well as up to 6 different colors simultaneously. The SH800 can accommodate interchangeable nozzle chips ranging in sizes from 70 to 130 microns and are able to analyze and sort cells at various pressures. Analysis occurs at the microfluidic sorting chip, increasing sensitivity and resolution.  Acceleration of the stream through the nozzle chip achieves droplet rates similar to that of jet-in-air sorters. Using a 100 micron nozzle chip, a sample can be analyzed at a rate of 10,000 events per second (36 million events per hour) and sorted with a purity of greater than 98%. Samples can be run at user-adjustable flow rates and sorts have been performed under various conditions resulting in viable cells that proliferated for several days post-sort. The sample chamber and collection tube holder can be temperature controlled and sorted samples can be collected in various tube types with up to 2 different populations sorted at one time.  In addition, the SH800 has a Sort Deposition System which allows for single cell sorting into plates (96 well plate max) or onto slides.  For single cell sorting, there is also an option of Index Sorting, allowing one to record the fluorescence levels of the one sorted cell, and tie that data with any downstream application.  The SH800 in the Core is equipped with an Aerosol Management Option (AMO) which contains aerosols that are generated during a clog.  BSL-2 samples can be sorted on this instrument.  The sorter is operated using Sony SH800 Software and is available for all labs to use.

This service is highly recommended for those who sort using 6 colors or less and those labs using fluorescent proteins. If certain laser lines are required, we’ll help you make a recommendation on which instrument would be best for your situation.

SH800 chip

Training:  All SH800 users need to fulfill Basic Training requirements. Click HERE.

Assistance:  If you run samples infrequently, it is recommended to request Assistance to have a Flow Core staff member run samples for you. Click HERE.

Rates:  Click HERE.

Scheduling:  Click HERE.

Data Analysis:  Raw data is in a FCS file format.  For analysis options, click HERE.

Setup & Operation:  During normal SH800 working hours of Monday-Friday, 8am-7pm, the instrument will be setup for you by Flow Core staff. “SH800 Setup” is defined as: chip aligned, stable stream breakoff set, machine QCd, drop delay calibrated, and sort streams aligned.  After setup has been performed, you can run samples yourself once you have completed Basic Training. If you are not a superuser and need assistance setting up the instrument during non-normal work hours (Monday-Friday from 7pm-8am, weekends, or holidays), you can request 1 hour of Assistance from a core staff member so we can get the instrument setup for you. If you sort frequently during non-normal work hours, it is highly recommended to go through the SH800 Advanced Training to become a superuser.  This instrument is much easier to setup compared to other cell sorters.  If you sort infrequently, it is recommended to use the MoFlo XDP for your cell sorting needs.

Instrument Features:  The Summary Table HERE will provide a matrix of all the key features of each instrument.  Click on the nickname link of a specific instrument to get a more detailed layout.  Tabs are also at the bottom listing the machine nicknames.  It is essential for planning your experiments and seeing if an instrument can detect your specific markers.  If you need to download a copy, click on "File" then "Download as" from the Google menu.  Check out what the instrument is equipped with and contact us if you have any questions about it.