Self Analysis on the LSR/Fortessa

LSRII Flow Cell

Availability: 24 hours, 7 days a week.

Overview:  The BD LSRII, Fortessa, and X-20 class of instruments are flow cytometer analyzers equipped with 4 or more lasers, generating data of up to 20 parameters for each event passing through the interrogation point.

The LSRII, Fortessa, and X-20 appear different, but the operation and workflow are performed in the same manner on all instrument types.  Some are also equipped to take the High Throughput Sampler (HTS) for 96 and even 384 well type plates.  Analysis occurs at the cuvette flow cell, increasing sensitivity and resolution, allowing detection of 2 relative physical properties as well as up to 18 different colors simultaneously. It can be run at 3 different sample flow rates with a fine-tune adjustor and is capable of analysis rates of up to 40,000 events per second.  They are all operated using FACSDiva Software and are available for all labs to use.


Training:  All LSRII/Fortessa users need to fulfill Basic Training requirements.  Click HERE.

Assistance:  If you run samples infrequently, it is recommended to request Assistance to have a Flow Core staff member run samples for you. Click HERE.

Rates:  Click HERE. 

Scheduling:  Click HERE.

Data Analysis:  Raw data is in a FCS file format.  For analysis options, click HERE.


Instrument Features:  The instruments differ slightly in their laser excitation lines, detector capabilities, and whether or not they have an HTS to run plates. The Summary Table HERE will provide a matrix of all the key features of each instrument.  Click on the nickname link of a specific instrument to get a more detailed layout.  Tabs are also at the bottom listing the machine nicknames.  It is essential for planning your experiments and seeing if an instrument can detect your specific markers.  If you need to download a copy, click on "File" then "Download as" from the Google menu.  Check out what the instrument is equipped with and contact us if you have any questions about it.