Self Analysis on the FACSCalibur

FACSCalibur Flow Cell

Availability: 24 hours, 7 days a week.

Overview:  The BD FACSCalibur is a flow cytometer analyzer equipped with 2 lasers, generating data of up to 6 parameters for each event passing through the interrogation point.

Analysis occurs at the cuvette flow cell, increasing sensitivity and resolution, allowing detection of 2 relative physical properties as well as up to 4 different colors simultaneously. It can be run at 3 different sample flow rates and is capable of analysis rates of up to 20,000 events per second.  It is operated using CellQuest Pro Software and is available for all labs to use.

Training:  All FACSCalibur users need to fulfill Basic Training requirements.  Click HERE.

Assistance:  If you run samples infrequently, it is recommended to request Assistance to have a Flow Core staff member run samples for you. Click HERE.

Rates:  Click HERE.

Scheduling:  Click HERE.

Data Analysis:  Raw data is in a FCS file format.  For analysis options, click HERE.

Instrument Features:  The Summary Table HERE will provide a matrix of all the key features of each instrument.  Click on the nickname link of a specific instrument to get a more detailed layout.  Tabs are also at the bottom listing the machine nicknames.  It is essential for planning your experiments and seeing if an instrument can detect your specific markers.  If you need to download a copy, click on "File" then "Download as" from the Google menu.  Check out what the instrument is equipped with and contact us if you have any questions about it.