Self Sorting on the FACSAria

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Availability: 24 hours, 7 days a week. (Monday-Friday, 8am-7pm with "Aria-Setup.")

Overview:  The BD FACSAria2 SORP, FACSAria3u, and FACSAria Fusion are flow cytometer cell sorters equipped with 4 or more lasers, generating data of up to 20 parameters for each event passing through the interrogation point, and allows the collection of up to 4 different populations.

Although the various Aria models might look slightly different on the outside, their setup and operation is the same. These sorters can detect 2 relative physical properties as well as up to 18 different colors simultaneously. The FACSAria Fusion has an additional small particle detector for analysis and sorting of particles down to 200nm.  The Arias accommodate interchangeable nozzles ranging in sizes from 70 to 130 microns and are able to analyze and sort cells at various pressures. Analysis occurs at the cuvette flow cell, increasing sensitivity and resolution. Acceleration of the stream through the nozzle achieves droplet rates similar to that of jet-in-air sorters. Using a 70 micron nozzle, a sample can be analyzed at a rate of 25,000 events per second (90 million events per hour) and sorted with a purity of greater than 98%. Samples can be run at user-adjustable flow rates and sorts have been performed under various conditions resulting in viable cells that proliferated for several days post-sort. The sample chamber and collection tube holder can be temperature controlled and sorted samples can be collected in various tube types with up to 4 different populations sorted at one time.  In addition, all Arias in the Core have an Automated Cell Deposition Unit (ACDU) which allows for single cell sorting into plates (384 well plate max) or onto slides.  For single cell sorting, there is also an option of Index Sorting, allowing one to record the fluorescence levels of the one sorted cell, and tie that data with any downstream application. All Arias in the Core are equipped with an Aerosol Management Option (AMO) which contains aerosols that are generated during a clog.  BSL-2 samples can be sorted on these instruments.  The Arias are operated using FACSDiva Software and are available for all labs to use.

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Training:  All Aria users need to fulfill Basic Training requirements.  Click HERE.

Assistance:  If you run samples infrequently, it is recommended to request Assistance to have a Flow Core staff member run samples for you. Click HERE.  

Rates:  Click HERE. 

Scheduling:  Click HERE.

Data Analysis:  Raw data is in a FCS file format. For analysis options, click HERE.

Setup and Operation:  During normal Aria working hours of Monday-Friday, 8am-7pm, the Arias will be setup for you by Flow Core staff based on your needs documented on the scheduler. “Aria-Setup” is defined as: proper nozzle inserted, stable stream breakoff set, machine QCd, drop delay calibrated, and sort streams aligned. After setup has been performed, you can run samples yourself once you have completed Basic Training. If you are not a superuser and need assistance setting up the instrument during non-normal work hours (Monday-Friday from 7pm-8am, weekends, or holidays), you can request 1 hour of Assistance from a core staff member so we can get the instrument setup for you. If you sort frequently during non-normal work hours, it is highly recommended to go through the Aria Advanced Training to become a superuser. If you sort infrequently, it is recommended to use the MoFlo XDP for your cell sorting needs.

Instrument Features:  The instruments differ slightly in their laser excitation lines and detector capabilities.  The Summary Table HERE will provide a matrix of all the key features of each instrument.  Click on the nickname link of a specific instrument to get a more detailed layout.  Tabs are also at the bottom listing the machine nicknames.  It is essential for planning your experiments and seeing if an instrument can detect your specific markers.  If you need to download a copy, click on "File" then "Download as" from the Google menu.  Check out what the instrument is equipped with and contact us if you have any questions about it.