The MoFlo XDPs can detect 2 relative physical properties as well as up to 15 different colors simultaneously. The XDPs accommodate interchangeable nozzles ranging in sizes from 50 to 200 microns and are able to analyze and sort cells at various pressures. Analysis occurs after the hydrodynamic focusing of the stream through the nozzle. The XDP electronics allow for nearly zero dead time, resulting in significantly less hard aborts compared to the Aria and other flow cytometers, allowing researchers to see almost everything in their sample. Using a 70 micron nozzle, a sample can be analyzed at a rate of 50,000 events per second (180 million events per hour) and sorted with a purity of greater than 98%. Samples can be run at adjustable flow rates and sorts have been performed under various conditions resulting in viable cells that proliferated for several days post-sort. The sample chamber and collection tube holder can be temperature controlled and sorted samples can be collected in various tube types with up to 4 different populations sorted at one time. The MoFlo XDPs are both equipped with a CyClone arm, allowing single cell sorting into plates (1536 well plate max) or onto slides as well as an Aerosol Evacuation System (AES) which contains aerosols that are generated during a clog. BSL-2 samples can be sorted on these instruments. The MoFlo XDPs are operated using Summit Software and are available for all labs to use.
Although you are not required to take any training, it is recommended to have some understanding of flow cytometry basics, so you can get the most use from this instrument and service. MoFlo XDP users will find the suggested useful in understanding how the instrument works. Contact us if you would like hands-on training, which is available for those who want to learn how to operate the instrument and learn more about it.
You must with Flow Core staff so we will have the necessary controls, which you will provide us, to setup the instrument correctly. If you do not provide the necessary controls discussed, we cannot guarantee that the machine will be setup correctly for your experiment.
ff at .a
Since the instrument requires extensive training and experience for proper setup, this service is provided with operator only. This service is highly recommended for those who sort infrequently. For those who sort frequently during non-normal work hours, we will attempt to accommodate you, but it is recommended to learn how to use the or the SH800. If certain laser lines are required, we’ll help you make a recommendation on which instrument would be best for your situation.
The will provide a matrix of all the key features of each instrument. Click on the nickname link of a specific instrument to get a more detailed layout. Tabs are also at the bottom listing the machine nicknames. It is essential for planning your experiments and seeing if an instrument can detect your specific markers. If you need to download a copy, click on "File" then "Download as" from the Google menu. Check out what the instrument is equipped with and if you have any questions about it.