Operator Assisted Cell Sorting on the MoFlo XDP

MoFlo XDP Yoda

Availability: Monday-Friday, 10am-6pm. Core Staff Operator must be present.   

Overview:  The Beckman Coulter MoFlo XDPs are flow cytometer cell sorters equipped with 3 or more lasers, generating data of up to 17 parameters for each event passing through the interrogation point, and allows the collection of up to 4 different populations.

The MoFlo XDPs can detect 2 relative physical properties as well as up to 15 different colors simultaneously.  The XDPs accommodate interchangeable nozzles ranging in sizes from 50 to 200 microns and are able to analyze and sort cells at various pressures. Analysis occurs after the hydrodynamic focusing of the stream through the nozzle.  The XDP electronics allow for nearly zero dead time, resulting in significantly less hard aborts compared to the Aria and other flow cytometers, allowing researchers to see almost everything in their sample. Using a 70 micron nozzle, a sample can be analyzed at a rate of 50,000 events per second (180 million events per hour) and sorted with a purity of greater than 98%. Samples can be run at adjustable flow rates and sorts have been performed under various conditions resulting in viable cells that proliferated for several days post-sort. The sample chamber and collection tube holder can be temperature controlled and sorted samples can be collected in various tube types with up to 4 different populations sorted at one time. The MoFlo XDPs are both equipped with a CyClone arm, allowing single cell sorting into plates (1536 well plate max) or onto slides as well as an Aerosol Evacuation System (AES) which contains aerosols that are generated during a clog.  BSL-2 samples can be sorted on these instruments. The MoFlo XDPs are operated using Summit Software and are available for all labs to use.

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Training:  Although you are not required to take any training, it is recommended to have some understanding of flow cytometry basics, so you can get the most use from this instrument and service. MoFlo XDP users will find the suggested Tutorials useful in understanding how the instrument works. Contact us if you would like hands-on training, which is available for those who want to learn how to operate the instrument and learn more about it.

Assistance:  You must contact and consult with Flow Core staff so we will have the necessary controls, which you will provide us, to setup the instrument correctly.  If you do not provide the necessary controls discussed, we cannot guarantee that the machine will be setup correctly for your experiment.

Rates:  Click HERE.

Scheduling:  Contact Flow Core staff at flowassist@ucsf.edu as soon as you make the instrument reservation so that Core staff have as much advanced notice as possible. See here for advanced notice requirements and response times.  Requests made less than the required advanced notice may not be able to be accommodated. Core staff will sign up on your behalf once you provide them the appropriate date, time, and billing info. If the Assistance can be fit in, the Core may charge the service at double the rate depending on the circumstances.  If you continue to schedule last minute without justifiable reason, you will be denied access to the facility.  The MoFlo XDP Calendar is all blocked off with these same instructions in the Comments field.  If you see a Core Staff member's name there, with these same instructions above, then the time is available. Click HERE for additional scheduling information.

Data Analysis:  Raw data is in a FCS file format.  For analysis options, click HERE.

Setup and Operation:  Since the instrument requires extensive training and experience for proper setup, this service is provided with operator only. This service is highly recommended for those who sort infrequently. For those who sort frequently during non-normal work hours, we will attempt to accommodate you, but it is recommended to learn how to use the Aria or the SH800. If certain laser lines are required, we’ll help you make a recommendation on which instrument would be best for your situation.

Instrument Features:  The Summary Table HERE will provide a matrix of all the key features of each instrument.  Click on the nickname link of a specific instrument to get a more detailed layout.  Tabs are also at the bottom listing the machine nicknames.  It is essential for planning your experiments and seeing if an instrument can detect your specific markers.  If you need to download a copy, click on "File" then "Download as" from the Google menu.  Check out what the instrument is equipped with and contact us if you have any questions about it.